Role of miR‐486‐5p in regulating renal cell carcinoma cell proliferation and apoptosis via TGF‐β–activated kinase 1. Issue 3 (9th December 2018)

• Record Type: Journal Article
• Authors: He, Yanfa; Liu, Jianzhen; Wang, Yongjun; Zhu, Xiaoli; Fan, Zhengchao; Li, Chongbin; Yin, Hang; Liu, Ying
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 3(2019)
• Page Start: 2954
• Page End: 2963
• Publication Date: 2018-12-09
• Subjects: apoptosis -- C‐C motif chemokine ligand 2 (CCL2) -- miR‐486‐5p -- renal cell carcinoma -- TGF‐β–activated kinase 1 (TAK1); Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26900
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Renal cell carcinoma (RCC) is a common kidney tumor in adults. The role of miR‐486‐5p in RCC is unknown. The aim of our study was to identify new targets regulated by miR‐486‐5p in RCC, to obtain a deeper insight into the network and to better understand the role of these microRNAs and their targets in carcinogenesis of RCC. We performed a series of tests and found consistently lower expression levels of miR‐486‐5p in kidney cancer cells. Restoration of miR‐486‐5p expression in RCC cells could lead to the suppression of cell proliferation and the increase of cell apoptosis. Further studies demonstrated that TGF‐β–activated kinase 1 was a target gene of miR‐486‐5p in kidney cancer cells. It was also shown that C‐C motif chemokine ligand 2 (CCL2) from tumor‐associated macrophages downregulated miR‐486‐5p expression, and miR‐486‐5p inhibited RCC cell proliferation and apoptosis resistance induced by CCL2. The study demonstrates that there are potential diagnosis and therapy values of miR‐486‐5p in RCC. Abstract : Renal cell carcinoma (RCC) with low levels of miR‐486‐5p expression compared with normal tissues have a relatively poor prognosis, whereas high miR‐486‐5p expression is associated with a long survival rate in patients with RCC. TGF‐β–activated kinase 1 (TAK1) was a target gene of miR‐486‐5p in RCC cells. Low levels of miR‐486‐5p were negatively associated with TAK1 in patients with RCC . miR‐486‐5p could suppress RCC cell proliferation and increase cell apoptosis. C‐C motif chemokine ligand 2 (CCL2) from tumor‐associated macrophages downregulated miR‐486‐5p levels in RCC cells and miR‐486‐5p inhibited cell proliferation and induced apoptosis of RCC cells in the presence of CCL2.