Assessment of cellular and serum proteome from tongue squamous cell carcinoma patient lacking addictive proclivities for tobacco, betel nut, and alcohol: Case study. Issue 7 (25th March 2018)

• Record Type: Journal Article
• Authors: Khowal, Sapna; Naqvi, Samar H.; Monga, Seema; Jain, Swatantra K.; Wajid, Saima
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 7(2018)
• Page Start: 5186
• Page End: 5221
• Publication Date: 2018-03-25
• Subjects: oral cancer -- OSCC -- proteomics -- real time PCR; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26554
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: The intriguing molecular pathways involved in oral carcinogenesis are still ambiguous. The oral squamous cell carcinoma (OSCC) ranks as the most common type constituting more than 90% of the globally diagnosed oral cancers cases. The elevation in the OSCC incidence rate during past 10 years has an alarming impression on human healthcare. The major challenges associated with OSCC include delayed diagnosis, high metastatic rates, and low 5‐year survival rates. The present work foundations on reverse genetic strategy and involves the identification of genes showing expressional variability in an OSCC case lacking addictive proclivities for tobacco, betel nut, and/or alcohol, major etiologies. The expression modulations in the identified genes were analyzed in 16 patients comprising oral pre‐cancer and cancer histo‐pathologies. The genes SCCA1 and KRT1 were found to down regulate while DNAJC13, GIPC2, MRPL17, IG ‐ Vreg, SSFA2, and UPF0415 upregulated in the oral pre‐cancer and cancer pathologies, implicating the genes as crucial players in oral carcinogenesis. Abstract : Relative quantity of selected genes in oral precancerous and cancer pathology. POB, patient oral biopsy (Red); ANT, adjacent normal tissue (Blue). Conditions—I: inflammation; II: squamous papilloma; IV: dysplasia; V: severe dysplasia; VII: OSCC‐WD; VIII: OSCC‐MD. The black color dots represent precancer and/or cancer lesions showing relative expression trend different from the non addicted case.