Chemerin/ChemR23 axis triggers an inflammatory response in keratinocytes through ROS‐sirt1‐NF‐κB signaling. Issue 4 (13th November 2018)

• Record Type: Journal Article
• Authors: Wang, Yuan; Huo, Jia; Zhang, Dingwei; Hu, Gang; Zhang, Yanfei
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 4(2019)
• Page Start: 6459
• Page End: 6470
• Publication Date: 2018-11-13
• Subjects: chemerin -- inflammatory response -- keratinocytes -- p65 acetylation -- psoriasis -- sirt1; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.27936
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Psoriasis is a chronic disease which carries the emotional and social burden, promotes joint disability and raises comorbidity possibility in patients. Obesity is closely correlated with the occurrence of psoriasis and adipokines produced by adipose tissues were found to be critical culprits. Chemerin is one of them and its expression was increased in patients with psoriatic arthritis. In our hypothesis, chemerin might act on keratinocytes and promote an inflammatory response, which plays an essential role in psoriatic epidermis. To validate our hypothesis, HaCaT cells and primary human keratinocytes were treated with chemerin (5, 10, and 20 ng/mL for 24 hours). Enzyme‐linked immunosorbent assay (ELISA) was used to determine the secretion of inflammatory factors. Nuclear factor‐κB (NF‐κB) activation and p65 acetylation were evaluated by Western blot analysis. The expression and activity of sirtuin 1 (sirt1), a deacetylase act on p65, were also analyzed. The results showed that chemerin prompted inflammatory factors secretion, NF‐κB activation and p65 acetylation through chemerin receptor 23 receptor. Chemerin constrained the expression and deacetylase activity of sirt1 through augment of reactive oxygen species (ROS) production. Additionally, chemerin exacerbated psoriasiform dermatitis in imiquimod‐treated mice model. In conclusion, chemerin can seduce inflammatory response and promote NF‐κB activation through inhibition of sirt1 activity by ROS production. Abstract : In conclusion, chemerin can seduce inflammatory response and promote nuclear factor‐κB (NF‐κB) activation through inhibition of sirtuin 1 (sirt1) activity by reactive oxygen species (ROS) production