GHSR DNA hypermethylation is a new epigenetic biomarker for gastric adenocarcinoma and beyond. Issue 9 (24th January 2019)

• Record Type: Journal Article
• Authors: Amini, Mohammad; Foroughi, Kobra; Talebi, Fatemeh; Aghagolzade Haji, Hemat; Kamali, Fatemeh; Jandaghi, Pouria; Hoheisel, Jörg D.; Manoochehri, Mehdi
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 9(2019:Sep.)
• Page Start: 15320
• Page End: 15329
• Publication Date: 2019-01-24
• Subjects: biomarker -- DNA methylation -- gastric cancer -- GHSR; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28179
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Aberrations of DNA methylation are early events in the development of tumors. In this study, we investigated the DNA methylation status of growth hormone secretagogue receptor ( GHSR), a promising pan‐cancer biomarker, in gastric cancer (GC). Initially, data sets from DNA methylation and gene expression studies available at Gene Expression Omnibus (GEO) were analyzed. Confirmation was done on primary tumor specimens and adjacent normal stomach tissue samples. Both analyses showed significant hypermethylation of GHSR . For further validation, The Cancer Genome Atlas data on stomach cancer was used. A receiver operating characteristic curve analysis yielded an area under the curve value of 0.85, corroborating its usefulness as a diagnostic marker. A genome‐wide comethylation analysis revealed several correlated genes. CREB1 was found to act as an upstream regulator of this gene network. Furthermore, GHSR methylation was found to be a biomarker in several other tumor entities, namely cancers of the bladder, endometrium, esophagus, head and neck, liver, thyroid, kidney, and ovary. Our findings along with previous reports on other types of cancer suggest a high potential of GHSR gene methylation as a pan‐cancer biomarker, which could be considered for liquid biopsy applications. Abstract : We investigated the DNA methylation status of growth hormone secretagogue receptor (GHSR), a promising pan‐cancer biomarker, in gastric cancer (GC) using several data sets. Our findings along with previous reports on other types of cancer suggest a high potential of GHSR gene methylation as a pan‐cancer biomarker, which could be considered for liquid biopsy applications.