Current approaches in identification and isolation of cancer stem cells. Issue 9 (11th February 2019)

• Record Type: Journal Article
• Authors: Akbarzadeh, Maryam; Maroufi, Nazila Fathi; Tazehkand, Abbas Pirpour; Akbarzadeh, Moloud; Bastani, Sepideh; Safdari, Reza; Farzane, Ali; Fattahi, Amir; Nejabati, Hamid Reza; Nouri, Mohammad; Samadi, Nasser
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 9(2019:Sep.)
• Page Start: 14759
• Page End: 14772
• Publication Date: 2019-02-11
• Subjects: aldehyde dehydrogenase -- cancer stem cell -- side population -- spheroid formation assay -- surface marker; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28271
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Cancer stem cells (CSCs) are tumor cells with initiating ability, self‐renewal potential, and intrinsic resistance to conventional therapeutics. Efficient isolation and characterization of CSCs pave the way for more comprehensive knowledge about tumorigenesis, heterogeneity, and chemoresistance. Also a better understanding of CSCs will lead to novel era of both basic and clinical cancer research, reclassification of human tumors, and development of innovative therapeutic strategies. Finding novel diagnostic and effective therapeutic strategies also enhance the success of treatment in cancer patients. There are various methods based on the characteristics of the CSCs to detect and isolate these cells, some of which have recently developed. This review summarized current techniques for effective isolation and characterization of CSCs with a focus on advantages and limitations of each method with clinical applications. Abstract : This review summarized current techniques for effective isolation and characterization of cancer stem cells with a focus on advantages and limitations of each method with clinical applications.