Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses. (28th May 2021)

• Record Type: Journal Article
• Authors: Yang, Xiaojuan; Le Corronc, Hervé; Legendre, Pascal; Triller, Antoine; Specht, Christian G
• Is Part Of: EMBO reports. Volume 22:Number 7(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-28
• Subjects: direct stochastic optical reconstruction microscopy -- GABAAR -- GlyR -- single‐molecule localization microscopy -- sub‐synaptic domains; Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.202052154
• Languages: English
• ISSNs: 1469-221X

Abstract

Abstract: Super‐resolution imaging has revealed that key synaptic proteins are dynamically organized within sub‐synaptic domains (SSDs). To examine how different inhibitory receptors are regulated, we carried out dual‐color direct stochastic optical reconstruction microscopy (dSTORM) of GlyRs and GABAA Rs at mixed inhibitory synapses in spinal cord neurons. We show that endogenous GlyRs and GABAA Rs as well as their common scaffold protein gephyrin form SSDs that align with pre‐synaptic RIM1/2, thus creating trans‐synaptic nanocolumns. Strikingly, GlyRs and GABAA Rs occupy different sub‐synaptic spaces, exhibiting only a partial overlap at mixed inhibitory synapses. When network activity is increased by 4‐aminopyridine treatment, the GABAA R copy numbers and the number of GABAA R SSDs are reduced, while GlyRs remain largely unchanged. This differential regulation is likely the result of changes in gephyrin phosphorylation that preferentially occurs outside of SSDs. The activity‐dependent regulation of GABAA Rs versus GlyRs suggests that different signaling pathways control the receptors' sub‐synaptic clustering. Taken together, our data reinforce the notion that the precise sub‐synaptic organization of GlyRs, GABAA Rs, and gephyrin has functional consequences for the plasticity of mixed inhibitory synapses. SYNOPSIS: This study analyzes the internal organization of mixed inhibitory spinal cord synapses using dSTORM and reveals the existence of trans‐synaptic nanocolumns containing GlyRs and GABAA Rs that are differentially regulated in response to altered network activity. Sub‐synaptic domains (SSDs) are aligned in trans‐synaptic nanocolumns at inhibitory synapses. SSDs formed by GlyRs and GABAA Rs are differentially spatially organized at mixed inhibitory synapses. GABAA Rs but not GlyRs show activity‐dependent regulation at mixed inhibitory synapses. Gephyrin phosphorylation is compartmentalized in SSDs within the synaptic scaffold. Abstract : This study analyzes the internal organization of mixed inhibitory spinal cord synapses using dSTORM and reveals the existence of trans‐synaptic nanocolumns containing GlyRs and GABAA Rs that are differentially regulated in response to altered network activity.