YAP1 regulates PPARG and RXR alpha expression to affect the proliferation and differentiation of ovine preadipocyte. Issue 12 (11th July 2019)

• Record Type: Journal Article
• Authors: Deng, Kaiping; Ren, Caifang; Fan, Yixuan; Pang, Jing; Zhang, Guomin; Zhang, Yanli; You, Peihua; Wang, Feng
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 12(2019)
• Page Start: 19578
• Page End: 19589
• Publication Date: 2019-07-11
• Subjects: adipogenesis -- adipose tissue development -- Hippo pathway -- YAP1; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.29265
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Adipose tissue development is regulated by a serial of developmental signaling pathways. The Hippo pathway is a novel signaling cascade closely associated with adipogenesis. While most of Hippo pathway components had been verified that have a vital role in preadipocytes proliferation and differentiation, little is known about the function of Yes‐associated protein 1 (YAP1) in mammalian adipose tissue development. Therefore, we investigated the role of YAP1 in ovine adipose tissue development by in vitro and in vivo experiments. We observed that the adipocyte size in subcutaneous adipose tissue increased with development. YAP1 expression increased during adipose tissue development, while decreased during the differentiation of ovine preadipocytes in vitro. YAP1 knockdown notably promoted lipid accumulation and suppressed ovine preadipocyte proliferation. In addition, we observed that YAP1 deficiency significantly upregulated peroxisome proliferator‐activated receptor gamma (PPARG) and retinoid X receptor alpha (RXR alpha) expression. By contrast, overexpression of YAP1 led to the suppression of preadipocyte differentiation, lipid droplets formation, and PPARG expression. In brief, our findings demonstrated that YAP1 regulates the proliferation and differentiation of ovine preadipocyte via altering PPARG and RXR alpha expression. Abstract : Adipocyte size in subcutaneous adipose tissue of sheep increased with development. Yes‐associated protein 1 regulates the proliferation and differentiation of ovine preadipocyte via altering peroxisome proliferator‐activated receptor gamma and retinoid X receptor alpha expression