Circ_0011460 upregulates HTRA1 expression by sponging miR‐762 to suppress HTR8/SVneo cell growth, migration, and invasion. (26th July 2021)

• Record Type: Journal Article
• Authors: Fan, Zhongyan; Wang, Qiming; Deng, Hui
• Is Part Of: American journal of reproductive immunology. Volume 86:Number 5(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-26
• Subjects: circ_0011460 -- HTR8/SVneo cells -- HTRA1 -- miR‐762 -- preeclampsia; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.13485
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract: Background: Aberrant expression of circular RNAs (circRNAs) during placental development could affect fetal growth and contribute to preeclampsia (PE). Circ_0011460 was found to be differentially expressed in placental samples of PE. However, the exact function and mechanism of circ_0011460 in PE process remain largely undefined. Methods: Levels of circ_0011460, microRNA (miR)‐762, and high‐temperature requirement‐A serine peptidase 1 (HTRA1) were detected using quantitative real‐time polymerase chain reaction and Western blot. In vitro experiments in HTR8/SVneo cells were conducted using cell counting kit‐8, wound healing, transwell, flow cytometry and Western blot assays. The direct interactions between miR‐762 and circ_0011460 or HTRA1 were verified using dual‐luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull‐down assays. Results: Circ_0011460 possessed a loop structure and was highly expressed in placental tissues of PE patients. Overexpression of circ_0011460 greatly suppressed HTR8/SVneo cell proliferation, migration, and invasion, and accelerated cell apoptosis. While circ_0011460 knockdown yielded the opposite trends on above biological behaviors. Mechanistically, we confirmed that circ_0011460 could up‐regulate HTRA1 expression via serving as a sponge of miR‐762. Further rescue studies demonstrated that circ_0011460 exerted its roles via targeting miR‐762, and miR‐762 promoted HTR8/SVneo cell growth, migration and invasion via regulating HTRA1. Conclusion: In all, circ_0011460 suppressed HTR8/SVneo cell growth, migration, and invasion via miR‐762/HTRA1 axis, suggesting a new insight into the pathogenesis of PE.