Resorc[4]arene Modifiers for Supramolecular Site‐Directed Immobilization of Antibodies on Multi‐Walled Carbon Nanotubes. (4th April 2023)

• Record Type: Journal Article
• Authors: Polli, Francesca; Cianfoni, Gabriele; Elnahas, Rem; Mangiardi, Laura; Scaramuzzo, Francesca A.; Cammarone, Silvia; Quaglio, Deborah; Calcaterra, Andrea; Pierini, Marco; Mazzei, Franco; Zanoni, Robertino; Botta, Bruno; Ghirga, Francesca
• Is Part Of: Chembiochem. Volume 24:Number 9(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-04-04
• Subjects: biosensor -- carbon nanotubes -- resorc[4]arenes -- SARS-CoV-2 -- site-directed immobilization; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202300030
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: One of the main problems in developing immunosensors featuring carbon nanotubes (CNTs) is immobilizing antibodies (Abs) onto the CNT surface to afford selective binding to target antigens (Ags). In this work, we developed a practical supramolecular Ab conjugation strategy based on resorc[4]arene modifiers. To improve the Ab orientation on the CNTs surface and optimizing the Ab/Ag interaction, we exploited the host‐guest approach by synthesizing two newly resorc[4]arene linkers R1 and R2 via well‐established procedures. The upper rim was decorated with eight methoxyl groups to promote selective recognition of the fragment crystallizable (Fc ) region of the Ab. Moreover, the lower rim was functionalized with 3‐bromopropyloxy or 3‐azidopropiloxy substituents to bind the macrocycles on the multi‐walled carbon nanotubes (MWCNTs) surface. Accordingly, several chemical modifications of MWCNTs were evaluated. After the morphological and electrochemical characterization of nanomaterials, the resorc[4]arene‐modified MWCNTs were deposited onto a glassy carbon electrode surface to evaluate their potential applicability for label‐free immunosensor development. The most promising system showed an improved electrode active area (AEL ) of almost 20 % and a site‐oriented immobilization of the SARS‐CoV‐2 spike protein S1 antibody (Ab‐SPS1). The developed immunosensor revealed a good sensitivity (23.64 μA mL ng −1 cm −2 ) towards the SPS1 antigen and a limit of detection (LOD) of 1.01 ng mL −1 . Abstract : Carbon nanotubes were covalently decorated with resorc[4]arenes to improve the site‐direct immobilization of SPS1 antibody . This system was used to fabricate an immunosensor for the detection of SPS1 from SARS‐CoV‐2.