Lnc‐CPLC promotes the progression of colorectal cancer via regulating ZBTB34 by competitively binding miR‐4319. Issue 2 (6th November 2021)

• Record Type: Journal Article
• Authors: Liao, Jingnan; Liu, Jinglan; Wang, Jinjin; Wang, Mingyuan
• Is Part Of: Journal of cellular physiology. Volume 237:Issue 2(2022)
• Page Start: 1573
• Page End: 1585
• Publication Date: 2021-11-06
• Subjects: colorectal cancer (CRC) -- CPLC -- LncRNA -- miR4319 -- ZBTB34; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.30628
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Long noncoding RNAs (lncRNAs) have been considered as regulatory molecules that play crucial roles in diverse biological processes, including the regulation of tumor progression. However, in colorectal cancer (CRC), due to the complex regulatory relationships involved in lncRNAs, the details of the specific mechanism still need to be elucidated. To discover the key regulatory role of lncRNA in CRC, we used bioinformatics analysis methods for preliminary screening. Through the combination of in vitro and in vivo verification, we further comprehensively analyzed the specific regulation of the key gene and the related key lncRNA in CRC. We found that ZBTB34 and lnc‐CPLC (CRC progression‐associated lncRNA) had a strong correlation, which plays a key role in the regulation of CRC. Furthermore, by exerting the "sponge" function, lnc‐CPLC could bind to miR4319 and release its binding to the 3'UTR of ZBTB34 mRNA. Our results reveal the mechanism of the lnc‐CPLC/miR‐4319/ZBTB34 signal axis in CRC and provide evidence for elucidating the complex molecular mechanisms in tumors. Abstract : In this study, we identified a lncRNA (long intergenic non‐protein coding RNA 893, lnc00893), which we named CRC progression‐associated lncRNA (CPLC), could be recognized as a competitive endogenous RNA, which binds to miR‐4319 through the "sponge" function to promote the expression of ZBTB34. This study reveals the regulatory role of lnc‐CPLC/miR‐4319/ZBTB34 signal axis in CRC and provides an important basis for improving the research of lncRNAs in tumors.