Chemical approaches for the preparation of ubiquitinated proteins via natural linkages. (12th September 2021)

• Record Type: Journal Article
• Authors: Zhou, Yuhui; Xie, Qingsong; Wang, Huagui; Sun, Hao
• Is Part Of: Journal of peptide science. Volume 28:Number 3(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-12
• Subjects: chemical protein synthesis -- isopeptide bond -- semi‐synthesis -- ubiquitinated protein -- ubiquitination; Peptides -- Periodicals; Peptides -- Periodicals; 572.65
• DOI: 10.1002/psc.3367
• Languages: English
• ISSNs: 1075-2617

Abstract

Abstract : Ubiquitination is an important posttranslation modification (PTM) that regulates a variety of cellular processes, including protein degradation, DNA repair, and viral infections. In this process, the C‐terminal carboxyl group of ubiquitin (Ub) or poly‐Ub is attached to the ε‐amine of lysine (Lys) side chain of an acceptor protein through an isopeptide bond. Studying a molecular mechanism of ubiquitination and deubiquitination is fundamental for unraveling its precise role in health and disease and hence crucial for drug development. Enzymatic approaches for protein ubiquitination possess limited ability to selectivity install Ub or Ub chain on the desired position of an acceptor protein and often lead to heterogeneous mixtures. In the past decades, chemical protein (semi)synthesis has been proved to be an efficient tool to facilitate site‐specific protein ubiquitination, which significantly contributes to decode the Ub signal at molecular and structural levels. In this review, we summarize the synthetic strategies developed for protein ubiquitination, and the achievements to generate monoubiquitinated, di‐ubiquitinated, and tetraubiquitinated proteins with native isopeptide and ester bonds. Abstract : This review summarizes the most widely used chemical synthetic strategies developed for protein ubiquitination, and the achievements to generate mono‐, di‐ and tetra‐ubiquitinated proteins with native isopeptide and ester bonds.