A novel magnetic fluid for ultra-fast and highly efficient extraction of N1-methylnicotinamide in urine samples. (4th April 2023)

• Record Type: Journal Article
• Authors: Chen, Zhuhui; Xiong, Yue; Ma, Ranran; Chen, Pei; Duan, Le; Yang, Shuying; Gisèle, Ineza Urujeni; You, Linjun; Xiao, Deli
• Is Part Of: New journal of chemistry. Volume 47:Number 16(2023)
• Page Start: 7862
• Page End: 7872
• Publication Date: 2023-04-04
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d3nj00488k
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Among the three pre-treatment materials, Fe3 O4 @HPMC@DMSA NPs were selected to be the best material and were used to perform MSPE-HPLC-UV. Abstract : To overcome the low concentration and matrix complexity of N 1 -methylnicotinamide (MeNam) in biological samples, this work developed a method for the detection of MeNam in urine samples using a Fe3 O4 @hydroxypropylmethylcellulose@2, 3-dimercaptosuccinic acid (Fe3 O4 @HPMC@DMSA) magnetic fluid as a pre-treatment material in combination with high performance liquid chromatography. The novel adsorbent material exhibited ultra-fast adsorption kinetics and a large adsorption capacity because Fe3 O4 @HPMC@DMSA NPs acted as a homogeneous magnetic fluid with more varieties and quantities of interaction sites (hydrogen-bonding interaction and electrostatic interaction). The limit of determination for MeNam was 0.05 μg mL −1 with Fe3 O4 @HPMC@DMSA NPs. Recoveries of spiked urine samples ranged between 95.49% and 106.4%, indicating that the developed method was promising for the accurate quantification of trace amounts of MeNam in urine samples. It provides research value for the rapid assessment of the nutritional effects of NMN dietary supplements and offers a sensitive assay for metabolism studies of related functional foods.