Integrative analysis of microbiome and metabolome in rats with Gest-Aid Plus Oral Liquid supplementation reveals mechanism of its healthcare function. (6th August 2021)

• Record Type: Journal Article
• Authors: Cheng, Min; Sun, Yuanfang; Wang, Leqi; Tan, Lirong; Jin, Huizi; Yan, Shikai; Li, Shasha; Xiao, Xue
• Is Part Of: Food quality and safety. Volume 5(2021)
• Publication Date: 2021-08-06
• Subjects: Gest-Aid Plus Oral Liquid -- healthcare function -- microbiomics -- metabolomics -- short-chain fatty acids; Food -- Periodicals; 641.3
• DOI: 10.1093/fqsafe/fyab010
• Languages: English
• ISSNs: 2399-1402

Abstract

Abstract: Objective: This study aimed to elucidate the possible mechanism of Gest-Aid Plus Oral Liquid (GAP) on healthcare function. Method: Ultrahigh-performance liquid chromatography–quadrupole time-of-flight mass spectrometry-based metabolomics and 16S rDNA sequencing of gut microbiota were performed on serum and fecal samples of GAP and control rats. Additionally, short-chain fatty acids (SCFAs) and inflammatory cytokines in fecal samples were determined through gas chromatography–mass spectrometry and enzyme-linked immunosorbent assay kits. Result: Metabolomics discovered 41 metabolites, which mainly involved amino acid metabolism, lipid metabolism, coenzyme factors, and vitamin metabolism. Administration of GAP increased abundance of Prevotella_9, Alloprevotella, Blautia, Phascolarctobacterium, Parabacteroides, and Fusicatenibacter, and six SCFAs were increased in the GAP group. Measurement of inflammatory cytokines showed that GAP had an anti-inflammatory effect in rats. Conclusion: Administration of GAP greatly affects the aspartate metabolism and microecology of rats, enhances intestinal motility and gut barrier integrity and anti-inflammation. These findings not only have possible implications for further application of GAP, but also provide a link between the gut microbiome, SCFAs, inflammation and serum metabolites in rats.