Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications. (12th April 2023)

• Record Type: Journal Article
• Authors: Dar, Amara; Ahmad, Muhammad Nayab; Samin, Ghufrana; Jahangir, Muhammad Muzammil; Rehman, Rabia; Anwar, Jamil; Al-thagafi, Zahrah T.; Meraf, Zelalem; Jaber, Mustafa Musa
• Other Names: Dar Alamgir Ahmad Academic Editor.
• Is Part Of: International journal of analytical chemistry. Volume 2023(2023)
• Publication Date: 2023-04-12
• Subjects: Chemistry, Analytic -- Periodicals; 543.05
• DOI: 10.1155/2023/9914633
• Languages: English
• ISSNs: 1687-8760

Abstract

Abstract : A novel pressurized flow system for circular thin-layer chromatography (PC-TLC) has been successfully established and employed for the separation of amino acids, dyes, and pigments for safe medical imaging applications. In this system, the mobile phase is applied to a regular TLC plate through the tube and needle of an intravenous infusion set. The needle was fused in a hole underneath the center of the plate, while the second side end of the tube was connected to a microburette containing the solvent. This new assembly proved itself better in terms of separation time (within 5 minutes) and controlled flow of the solvent and horizontal movement of analyte components over chromatograms with better separation and R f values (glutamine: 0.26, valine: 0.44, phenylalanine: 0.60, chlorophyll a: 0.52, chlorophyll b: 0.43, xanthophyll: 0.18, carotenoid: 0.97, and pheophytin: 0.60) when a number of samples of amino acids, dyes, and pigments were separated by the developed apparatus and the conventional TLC procedure. The developed method was found distinctly rapid, precise, and eco-friendly (less solvent consuming) as compared to traditional ascending TLC.