EFFECT OF ABDOMINAL IRRADIATION IN MICE MODEL OF INFLAMMATORY BOWEL DISEASE. Issue 6 (14th March 2023)

• Record Type: Journal Article
• Authors: Kang, Sohi; Son, Yeonghoon; Shin, In-Sik; Moon, Changjong; Lee, Min Y; Lim, Kyung S; Park, Su-Jin; Lee, Chang-Geun; Jo, Wol S; Lee, Hae-June; Kim, Joong S
• Is Part Of: Radiation protection dosimetry. Volume 199:Issue 6(2023)
• Page Start: 564
• Page End: 571
• Publication Date: 2023-03-14
• Subjects: Radiation dosimetry -- Periodicals; Radiation -- Safety measures -- Periodicals; 363.1799
• DOI: 10.1093/rpd/ncad051
• Languages: English
• ISSNs: 0144-8420

Abstract

Abstract: Inflammatory bowel diseases could be diagnosed in major measure by diagnostic imaging; however, radiation exposure in the intestine may also contribute to the progression of these pathologies. To better understand the impact of radiation in the presence of bowel disease, we administered dextran sodium sulfate (DSS) to C57BL/6 mice to induce colitis and exposed to radiation at abdominal area. We observed that abdominal irradiation (13 Gy) aggravates the DSS-induced decrease in survival rate (0%), body weight (74.54 ± 3.59%) and colon length (4.98 ± 0.14 cm). Additionally, abdominal irradiation markedly increased in colonic inflammation levels (3.16 ± 0.16) compared with that of DSS-induced sham mice. Furthermore, abdominal irradiation also increased the mRNA expression levels of inflammatory genes, such as cyclooxygenase-2 (13.10 folds), interleukin-6 (48.83 folds) and tumor necrosis factor-alpha (42.97 folds). We conclude that abdominal irradiation aggravates the detrimental effects of DSS-induced colitis in mice, which might be a useful guideline for inflammatory bowel disease patients.