The transcriptome of hand eczema assessed by tape stripping. (8th December 2021)

• Record Type: Journal Article
• Authors: Sølberg, Julie B. K.; Quaade, Anna S.; Jacobsen, Stine B.; Andersen, Jeppe D.; Kampmann, Marie‐Louise; Morling, Niels; Litman, Thomas; Thyssen, Jacob P.; Johansen, Jeanne D.
• Is Part Of: Contact dermatitis. Volume 86:Number 2(2022)
• Page Start: 71
• Page End: 79
• Publication Date: 2021-12-08
• Subjects: atopic hand eczema -- contact dermatitis -- subtypes of hand eczema -- tape stripping -- transcriptomics; Contact dermatitis -- Periodicals; 616.51
• DOI: 10.1111/cod.14015
• Languages: English
• ISSNs: 0105-1873

Abstract

Abstract: Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin‐tape strips for molecular investigations enable noninvasive investigations of HE. Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results: The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included epidermal biomarkers such as EPHA1 . Conclusion: Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes. Abstract : Whole transcriptome sequencing can be performed on RNA from tape strips from different localizations on the hands, from both hand eczema (HE) patients and healthy controls. The transcriptomic difference between HE with and without atopic dermatitis (AD) can be assessed by tape stripping and includes an increase of inflammatory markers for HE patients with AD. Skin tape strip samples identify transcriptomic differences between irritant and allergic contact dermatitis on the hands.