CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level. Issue 33 (4th April 2023)
- Record Type:
- Journal Article
- Title:
- CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level. Issue 33 (4th April 2023)
- Main Title:
- CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the single-cell level
- Authors:
- Zhao, Ning-ning
Tian, Xiaorui
Ma, Fei
Zhang, Chun-yang - Abstract:
- Abstract : We develop a new fluorescent biosensor for flap endonuclease 1 assay based on CRISPR/Cas12-enhanced single-molecule counting. Abstract : We develop a new fluorescent biosensor for flap endonuclease 1 (FEN1) assay based on CRISPR/Cas12-enhanced single-molecule counting. This biosensor is simple, selective, and sensitive with a detection limit of 2.325 × 10 −5 U and it is applicable for inhibitor screening, kinetic parameter analysis, and quantifying cellular FEN1 with single-cell sensitivity.
- Is Part Of:
- Chemical communications. Volume 59:Issue 33(2023)
- Journal:
- Chemical communications
- Issue:
- Volume 59:Issue 33(2023)
- Issue Display:
- Volume 59, Issue 33 (2023)
- Year:
- 2023
- Volume:
- 59
- Issue:
- 33
- Issue Sort Value:
- 2023-0059-0033-0000
- Page Start:
- 4939
- Page End:
- 4942
- Publication Date:
- 2023-04-04
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d3cc00642e ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 27072.xml