Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe. (2nd May 2012)

• Record Type: Journal Article
• Authors: Ramezani, Amir M.; Manzoori, Jamshid L.; Amjadi, Mohammad; Jouyban, Abolghasem
• Other Names: Montoliu Carmina Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2012(2012)
• Publication Date: 2012-05-02
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1100/2012/940541
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb 3+ -Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb 3+ -Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb 3+ -Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb 3+ ] = 8.5 × 10 - 5 mol L −1, [Dano] = 1.5 × 10 - 4 mol L −1 . The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2 × 10 − 6 − 1.3 × 10 − 6 mol L −1, 0.2 × 10 − 6 - 1.4 × 10 − 6 mol L −1, and 0.2 × 10 - 6 − 1 × 10 − 6 mol L −1, respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7 × 10 - 8 mol L −1, 6.2 × 10 − 8 mol L −1, and 8.1 × 10 − 8 mol L −1, respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.