Living and Injectable Porous Hydrogel Microsphere with Paracrine Activity for Cartilage Regeneration. Issue 17 (18th January 2023)

• Record Type: Journal Article
• Authors: Li, Xingchen; Li, Xiaoxiao; Yang, Jielai; Lin, Jiawei; Zhu, Yuan; Xu, Xiangyang; Cui, Wenguo
• Is Part Of: Small. Volume 19:Issue 17(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-18
• Subjects: hydrogel microspheres -- microfluidic -- osteoarthritis -- paracrine activity -- stem cell therapy; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202207211
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Paracrine is an important mechanism in mesenchymal stem cells (MSCs) that promotes tissue regeneration. However, anoikis is attributed to unsuitable adhesion microenvironment hindered this paracrine effect. In this study, a living and injectable porous hydrogel microsphere with long‐term paracrine activity is constructed via the freeze‐drying microfluidic technology and the incorporation of platelet‐derived growth factor‐BB (PDGF‐BB) and exogenous MSCs. Benefiting from the porous structure and superior mechanical property of methacrylate gelatin (GelMA) hydrogel microspheres (GMs), exogenous stem cells are able to adhere and proliferate on GMs, thereby facilitating cell‐to‐extracellular matrix (ECM) and cell‐to‐cell interactions and enhancing paracrine effect. Furthermore, the sustained release of PDGF‐BB can recruit endogenous MSCs to prolong the paracrine activity of the living GMs. In vitro and in vivo experiments validated that the living GMs exhibit superior secretion properties and anti‐inflammatory efficacy and can attenuate osteoarthritis (OA) progression by favoring the adherent microenvironment and utilizing the synergistic effect of exogenous and endogenous MSCs. Overall, a living injectable porous hydrogel microsphere that can enhance the paracrine activity of stem cells is fabricated and anticipated to hold the potential of future clinical translation in OA and other diseases. Abstract : The living hydrogel microspheres are constructed by incorporation of platelet‐derived growth factor‐BB (PDGF‐BB) and exogenous bone marrow mesenchymal stem cells with GelMA microspheres. The enhanced paracrine effect is achieved as the porous microspheres facilitated cell adhesion and proliferation; While the sustained release of PDGF‐BB enables long‐term paracrine activity by recruiting endogenous mesenchymal stem cells.