Changes in endogenous abscisic acid and stomata of the resurrection fern, Pleopeltis polypodioides, in response to de‐ and rehydration. Issue 4 (13th April 2023)

• Record Type: Journal Article
• Authors: John, Susan P.; Svihla, Zachary T.; Hasenstein, Karl H.
• Is Part Of: American journal of botany. Volume 110:Issue 4(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-04-13
• Subjects: abscisic acid -- calcium -- drought stress -- hydrogen peroxide -- nitric oxide -- Pleopeltis polypodioides -- stomata; Botany -- Periodicals; Botany; Electronic journals; Periodicals; 580
• DOI: 10.1002/ajb2.16152
• Languages: English
• ISSNs: 0002-9122

Abstract

Abstract: Premise: While angiosperms respond uniformly to abscisic acid (ABA) by stomatal closure, the response of ferns to ABA is ambiguous. We evaluated the effect of endogenous ABA, hydrogen peroxide (H2 O2 ), nitric oxide (NO), and Ca 2+, low and high light intensities, and blue light (BL) on stomatal opening of Pleopeltis polypodioides . Methods: Endogenous ABA was quantified using gas chromatography‐mass spectrometry; microscopy results and stomatal responses to light and chemical treatments were analyzed with Image J. Results: The ABA content increases during initial dehydration, peaks at 15 h and then decreases to one fourth of the ABA content of hydrated fronds. Following rehydration, ABA content increases within 24 h to the level of hydrated tissue. The stomatal aperture opens under BL and remains open even in the presence of ABA. Closure was strongly affected by BL, NO, and Ca 2+, regardless of ABA, H2 O2 effect was weak. Conclusions: The decrease in the ABA content during extended dehydration and insensitivity of the stomata to ABA suggests that the drought tolerance mechanism of Pleopeltis polypodioides is independent of ABA.