Comparison mitochondrial DNA DAMPs changes in platelet prepared by apheresis and PRP. Issue 3 (23rd November 2022)

• Record Type: Journal Article
• Authors: Chegini, Azita; Vakili, Leila; Samiee, Shahram; Shaiegan, Mojgan
• Is Part Of: Therapeutic apheresis and dialysis. Volume 27:Issue 3(2023)
• Page Start: 517
• Page End: 522
• Publication Date: 2022-11-23
• Subjects: apheresis -- blood platelets -- DNA -- mitochondrial -- platelet‐rich plasma; Hemapheresis -- Periodicals; Dialysis -- Periodicals; Blood Component Removal -- Periodicals; Renal Dialysis -- Periodicals; Hémaphérèse -- Périodiques; Dialyse -- Périodiques; Sang -- Collecte et conservation -- Périodiques; 616
• DOI: 10.1111/1744-9987.13950
• Languages: English
• ISSNs: 1744-9979

Abstract

Abstract: Introduction: Mt DNA (DAMPs) plays a key modulatory role in immune cells and may also mediate a variety of adverse transfusion reactions. Material and Method: The cross‐sectional study was performed on 22 (PRP) and 14 (SDP) between February 2019 and 2020. mtDNA DAMPs by quantitative real‐time PCR was assessed on days 1, 3, and 5 of platelets storage. The data was entered in REST 2009 software, and the amount of fold change was calculated. Multiple t tests were also used. Results: The mtDNA DAMPs fold change in SDP on days 1–3, 1.3 times, on days 3–5, 1.5 times, and on days 1–5, 2.1 times increased. The fold change on days 1–3, 0.8 times, on days 3–5, 0.6 times, and on days 1–5, 0.49 times decreased in PRP products. Conclusion: The method of preparation and processing can affect mtDNA DAMPs fold changes.