Heterogeneous tissue construction by on-demand bubble-assisted acoustic patterning. Issue 9 (3rd April 2023)

• Record Type: Journal Article
• Authors: Hu, Qinghao; Hu, Xuejia; Shi, Yang; Liang, Li; Zhu, Jiaomeng; Zhao, Shukun; Wang, Yifan; Wu, Zezheng; Wang, Fubing; Zhou, Fuling; Yang, Yi
• Is Part Of: Lab on a chip. Volume 23:Issue 9(2023)
• Page Start: 2206
• Page End: 2216
• Publication Date: 2023-04-03
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d3lc00122a
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : It offers a new strategy for the fabrication of functionalized structures for a variety of bionic tissue models. Abstract : Highly heterogeneous structures are closely related to the realization of the tissue functions of living organisms. However, precisely controlling the assembly of heterogeneous structures is still a crucial challenge. This work presents an on-demand bubble-assisted acoustic method for active cell patterning to achieve high-precision heterogeneous structures. Active cell patterning is achieved by the combined effect of acoustic radiation forces and microstreaming around oscillating bubble arrays. On-demand bubble arrays allow flexible construction of cell patterns with a precision of up to 45 μm. As a typical example, the in vitro model of hepatic lobules, composed of patterned endothelial cells and hepatic parenchymal cells, was constructed and cultured for 5 days. The good performance of urea and albumin secretion, enzymatic activity and good proliferation of both cells prove the feasibility of this technique. Overall, this bubble-assisted acoustic approach provides a simple and efficient strategy for on-demand large-area tissue construction, with considerable potential for different tissue model fabrication.