Endogenous glutathione-activated DNA nanoprobes for spatially controllable imaging of microRNA in living cells. Issue 36 (17th April 2023)

• Record Type: Journal Article
• Authors: Li, Yuyan; Zhang, Fanghua; Liu, Wendong; Shao, Mingzheng; Hao, Zhe; Zhang, Hongyan; Zhang, Ruizhong; Li, Xiyan; Zhang, Libing
• Is Part Of: Chemical communications. Volume 59:Issue 36(2023)
• Page Start: 5431
• Page End: 5434
• Publication Date: 2023-04-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d3cc01155k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A DNA nanoprobe, activated by glutathione (GSH), was designed to enable spatially selective sensing and imaging of miRNA in living cells. Abstract : A DNA nanoprobe, activated by glutathione (GSH), was designed to enable spatially selective sensing and imaging of miRNA in living cells. The nanoprobe was constructed using nano-sized metal–organic frameworks (MOFs) and DNA hairpin probes tethered to the surface of the MOFs, with the loop portion of the hairpin structure containing a disulfide bond. Cleavage of the disulfide bond by GSH triggers a strand-displacement reaction with target miRNAs, facilitating in situ readout of the fluorescence signal. The synergy of endogenous GSH activation and MOF improves the spatial resolution of miRNA detection and imaging.