Enantioselective effect of trifloxystrobin in early-stage zebrafish (Danio rerio) embryos: Cardiac abnormalities impacted by E, E-trifloxystrobin enantiomer. (15th June 2023)

• Record Type: Journal Article
• Authors: Kim, Yurim; Jeon, Hwang-Ju; Kim, Kyeongnam; Kim, Chaeeun; Moon, Joon-Kwan; Hwang, Kyu-Won; Lee, Sung-Eun
• Is Part Of: Environmental pollution. Volume 327(2023)
• Publication Date: 2023-06-15
• Subjects: E, E-trifloxystrobin -- Enantioselective toxicity -- Abnormal development -- Cardiac function failure -- Calcium voltage-gated channel; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121537
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Trifloxystrobin (TFS) is one of the extensively used strobilurin fungicides, which is composed of four enantiomers and its active form is E, E-TFS. In this study, we assess the acute toxicity of four enantiomers, E, E−, E, Z-, Z, E−, and Z, Z-TFS in zebrafish ( Danio rerio ) embryos. Among the four enantiomers, only E, E-TFS was found to be acutely toxic, with an estimated LC50 value of 0.68 mg/L. Treatment with E, E-TFS resulted in various phenotypic changes in the embryos, including pericardial and yolk-sac edema, spine curvature, and blood pooling. And it shortened the whole body length in the treated embryos by increasing the total intersegmental vessel numbers using a Tg(fli1a:EGFP) zebrafish line. Further study using Tg(cmlc2:EGFP) zebrafish line revealed that E, E-TFS treatment was associated with cardiac malformations, a failure of heart function, and a lowered heartbeat rate at the concentration of 0.25 mg/L. Also, the differential gene expression analysis identified significant down-regulation of vmhc and cacna1c genes encoding ventricular myosin heavy chain and calcium voltage-gated channel subunit alpha 1C, which are crucial for heart development. These results suggest the need for regular monitoring of E, E-TFS enantiomers after field application and further research into their potential chronic effects on environmental organisms. Graphical abstract: Image 1 Highlights: E, E-Trifloxystrobin (E, E-TFS) showed stronger acute toxicity to zebrafish embryos. Among four enantiomers, only E, E-TFS exhibited LC50 value of 0.68 mg/L. E, E-TFS caused morphological changes including pericardial and yolk-sac edemas. E, E-TFS induced abnormal cardiac formation with the failure of heart function.