Spent coffee grounds and orange peel residues based biorefinery for microbial oil and biodiesel conversion estimation. (June 2023)

• Record Type: Journal Article
• Authors: Giannakis, Nikos; Carmona-Cabello, Miguel; Makri, Aikaterini; Leiva-Candia, David; Filippi, Katiana; Argeiti, Chrysanthi; Pateraki, Chrysanthi; Dorado, M.P.; Koutinas, Apostolis; Stylianou, Eleni
• Is Part Of: Renewable energy. Volume 209(2023)
• Page Start: 382
• Page End: 392
• Publication Date: 2023-06
• Subjects: Spent coffee grounds -- Orange peel residues -- Microbial oil -- Biorefinery development -- Biodiesel; SCGs Spent coffee grounds -- OPR Orange peel residues -- FAMEs Fatty acid methyl esters -- TPC Total phenolic content -- MeO Methoxyl content -- AUA Total anhydrouronic acid content -- DE Degree of esterification -- PCA Principal component analysis -- UD Unsaturation degree -- LC Length of chain -- CN Cetane number -- LCV Lower calorific value -- CV Coefficient of variation -- CFPP Cold filter plugging point -- FP Flash point -- EW Equivalent weight; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2023.01.110
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: A consolidated biorefinery was developed using spent coffee grounds (SCGs) and orange peel residues (OPR) to produce microbial oil and value-added co-products. Coffee oil and antioxidant-rich extracts were initially extracted from SCGs. Free sugars and pectin-rich extracts were obtained from OPR. Microbial oil production was evaluated using various oleaginous yeast strains cultivated in commercial carbon sources. Microbial oil production was demonstrated in 30 L bioreactor scale using combined OPR-SCGs hydrolysate resulting in 56.7% (w/w) intracellular lipid accumulation and 0.32 g/(L∙h) productivity, corresponding to 68.1 g microbial oil production per 1 kg SCGs and 360 g OPR. Principal Component Analysis has been carried out to assess the similarity of microbial oil produced in this study and literature-cited oils considering the fatty acid methyl ester content. The biodiesel properties estimated based on predictive equations showed that they generally conformed with the limits set by European standards. Highlights: Spent coffee grounds and orange peel residues were converted into microbial oil. Biodiesel properties were estimated based on fatty acid profiles. Microbial oil production was efficiently demonstrated at 30 L bioreactor scale. Fermentation of OPR-SCGs hydrolysate led to 56.7% intracellular lipid accumulation. 68.1 g microbial oil were obtained from 1 kg SCGs and 360 g OPR.