MiR-22-3p modulated the antioxidant activity of curcumin via targeting the cardiolipin synthase gene CRLS1 in LO2 cells. (May 2023)

• Record Type: Journal Article
• Authors: Sun, Xiaoting; Li, Yuanyuan; Lin, Yanqi; Mei, Yuanyuan; Lin, Lingli; Ho, Kuo-Ting; Huang, Kaiyan; Zhan, Jialin; Chen, Chaoxiang; Zeng, Jun; Wu, Daren; Li, Jian; Liu, Jingwen; Li, Guiling
• Is Part Of: Journal of functional foods. Volume 104(2023)
• Publication Date: 2023-05
• Subjects: Antioxidation -- MicroRNAs -- Mitochondria -- Polyphenol -- Reactive oxygen species; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2023.105541
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Curcumin enhanced cellular antioxidant capacity via downregulating miR-22-3p. miR-22-3p reduced cellular antioxidant capacity via inhibiting CRLS1 expression. The redox regulation of miR-22-3p/ CRLS1 was attributed to mitochondrial modulation. Abstract: The antioxidant activity of curcumin has been extensively investigated for years. However, its molecular function mechanism remained unclear. Our preliminary study showed that curcumin downregulated miR-22-3p expression. This study aimed to address whether miR-22-3p mediated the antioxidant activity of curcumin and the possible action mechanism. The results showed that miR-22-3p repression increased the total cellular antioxidant capacity and enhanced the endogenous antioxidant enzyme system of LO2 cells. In addition, miR-22-3p-deteriorated cellular antioxidant capacity was prevented by curcumin pretreatment. Moreover, miR-22-3p inhibition significantly improved mitochondrial function and biogenesis. Further investigation demonstrated that a mitochondrial key regulator, the cardiolipin synthase gene ( CRLS1 ), was targeted and downregulated by miR-22-3p. CRLS1 overexpression also significantly improved cellular antioxidant capacity and enhanced mitochondrial function and biogenesis in LO2 cells. Taken together, all these data suggested that miR-22-3p modulated the antioxidant effect of curcumin via targeting CRLS1, which may provide novel insights into miRNA-mediated antioxidant mechanism of curcumin.