Aerobic biodegradation of trichloromethane by Stenotrophomonas sp. GYH and its biodegradation mechanism analysis. (May 2023)

• Record Type: Journal Article
• Authors: Cheng, Zhuowei; Shou, Deng; Zhao, Peng; Chen, Jianmeng; Zhao, Jingkai; Yu, Jianming; Zhang, Shihan; Guan, Yanhong
• Is Part Of: International biodeterioration & biodegradation. Volume 180(2023)
• Publication Date: 2023-05
• Subjects: TCM -- Aerobic biodegradation -- Dechlorination -- Genetic analysis -- Biodegradation pathway; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2023.105585
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: A new strain, Stenotrophomonas maltophilia GYH, used trichloromethane (TCM) as the only carbon source for its growth was isolated. This strain was the first reported bacterium with TCM aerobic degradation ability. The initial TCM concentration of 8.93 mg·L -1 resulted in a degradation efficiency of 100% and a mineralization efficiency of 52% under the role of the strain. Based on the carbon balance and chlorine balance, the strain GYH could convert about 40 μg organic C contained by TCM to 20.4 μg inorganic C and 19.4 μg C by biomass, along with the complete dechlorination of TCM in 84 h. The maximum specific growth and biodegradation rates were 0.078 and 0.018 h -1, respectively. Two TCM-degrading genes were cloned and successfully expressed in the constructed strains, with 66.45% and 23.61% dechlorination efficiency. In combination with gas and ion chromatography detection and genetic analysis, an aerobic biodegradation pathway of TCM was proposed. TCM was finally converted to CO2, Cl -, and biomass under the aerobic degradation of the GYH. Such results supply fundamental knowledge for future research to effectively explain the molecular mechanism of TCM aerobic biodegradation and expand the potential industrial application of TCM biodegradation in polluted environments. Graphical abstract: Image 1 Highlights: An aerobic strain was the first reported one that used TCM as the carbon source. The strain achieved 100% dechlorination and 52% mineralization for 8.93 mg·L -1 TCM. Two selected genes encoding dechlorination played roles for TCM biodegradation.