Novel peptide identified from viable-cell based phage display technique regulates growth cycle of Daphnia magna. (15th June 2023)

• Record Type: Journal Article
• Authors: Kim, Taehwan; Cho, Byung-Kwan; Kim, Yang-Hoon; Min, Jiho
• Is Part Of: Environmental pollution. Volume 327(2023)
• Publication Date: 2023-06-15
• Subjects: Daphnia magna asexual egg -- Phage display -- Specific target-binding peptides -- Teratogenic effect -- Multi-generational effect; Ala Alanine -- Arg Arginine -- Asn Asparagine -- Asp Aspartate -- FAM 5-Carboxyfluorescein -- Gln Glutamine -- Glu Glutamate -- Gly Glycine -- His Histidine -- HRW hard reconstituted water -- Ile Isoleucine -- IPTG isopropyl β-d-1-thiogalactopyranoside -- Leu Leucine -- Lys Lysine -- Met Methionine -- PEG poly(ethylene glycol) -- Phe Phenylalanine -- PI Propidium iodide -- Pro Proline -- RFI Relative Fluorescence Intensity -- Ser Serine -- TDW tertiary distilled water -- Thr Theonine -- Trp Tryptophan -- Tyr Tyrosine -- Val Valine -- X-gal 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121556
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Phage display is a widely used technique for selecting specific binding peptides, but presenting antigens in their natural form can be challenging, as protein coating may induce structural changes. In this study, we employed a whole cell-based phage display technique without a coating step to select peptides that bind specifically to Daphnia magna eggs. Boiled eggs were used as a control to ensure that antigens were presented in their natural forms. We identified a peptide, DEP1 (LYALPLSHLKSHGGG), with the highest binding affinity to D. magna eggs. DEP1 did not affect zebrafish eggs, but it inhibited normal hatching and reproductive ability in D. magna eggs, and hindered growth in neonates before their first ecdysis. Morphological analysis revealed that DEP1 caused intestinal damage and tissue abnormalities. Our findings demonstrate that the whole cell-based phage display technique is successful in presenting antigens in their natural form, and that the DEP1 peptide can be applied to regulate the growth cycle of D. magna . These results have implications for the use of phage display in environmental research and the potential use of DEP1 for hazardous organisms in aquatic systems. Graphical abstract: Image 1 Highlights: Viable egg specific binding sequence was identified by whole cell-based phage display. Sequence that distinguishes between viable and dead cells. A new whole cell-based phage display method that selects only viable eggs. The DEP1 peptide sequence selectively binds only to viable eggs. The DEP1 peptide sequence interferes with the normal growth of hatched individuals.