Increased neutrophil derived chemokines (CXCL10 and CCL2) in human trichinellosis as possible serological markers of the polarization of the immune response against the parasite. (June 2023)

• Record Type: Journal Article
• Authors: Bruschi, Fabrizio; Pinto, Barbara; Fallahi, Poupak; Ferrari, Silvia Martina; Antonelli, Alessandro
• Is Part Of: Cytokine. Volume 166(2023)
• Publication Date: 2023-06
• Subjects: Human trichinellosis -- CXCL10 -- CCL2 -- IL- 6 -- MMP-9 -- Clinical outcome; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2023.156205
• Languages: English
• ISSNs: 1043-4666

Abstract

Graphical abstract: Highlights: Immune response in human trichinellosis is characterized by a mixed polarization of Th1 and Th2 cells in line with the increase of CXCL10 and CCL2 chemokines. Among several cytokines studied in the patient sera, Il-6 levels resulted increased in almost half of the patients. CXCL10 levels are higher in patients with clinical signs typical of trichinellosis. CXCL10 and CCL2 are possible serological markers of the polarization of the immune response in trichinellosis. Abstract: Trichinella britovi is a widely distributed parasitic nematode, transmitted through ingestion of raw or poorly cooked meat containing muscle larvae. This helminth can regulate the host immune system during the early phase of infection. The immune mechanism mainly involves the interaction of Th1 and Th2 responses and related cytokines. Chemokines (C-X-C or C–C) and matrix metalloproteinases (MMPs) have also shown to be implicated in a number of parasitic infections, mainly malaria, neurocysticercosis, angiostronyloidosis, and schistosomiasis, but poor is known about their role in human Trichinella infection. We previously found that serum MMP-9 levels were significantly increased in T. britovi infected patients with relevant symptoms such as diarrhea, myalgia, and facial oedema, which makes these enzymes a potential reliable indicator of inflammation in trichinellosis patients. These changes were also observed in T. spiralis/T. pseudospiralis experimentally infected mice. No data are available about circulating levels of two pro-inflammatory chemokines, CXCL10 and CCL2, in trichinellosis patients with or w/o clinical signs of the infection. In this study, the association of serum level of CXCL10 and CCL2 with clinical outcome of T. britovi infection and their relation to MMP-9 were investigated. Patients (median age 49 ± 0.33 years) acquired infection by consuming raw sausages prepared with wild boar and pork meat. Sera were collected during the acute and the convalescent phases of the infection. A positive significant association (r = 0.61, p = 0.0004) was observed between MMP-9 and CXCL10 levels. The CXCL10 level significantly correlated with the severity of symptoms in patients being particularly higher in patients suffering diarrhea, myalgia, and facial oedema, thus suggesting a positive association of this chemokine with symptomatologic traits, especially myalgia (and increased LDH and CPK levels) ( p < 0.005). No correlation was found between levels of CCL2 and the clinical symptoms.