Identification of a novel fusion gene NLRC3-NLRP12 in miiuy croaker (Miichthys miiuy). Issue 136 (May 2023)

• Record Type: Journal Article
• Authors: Liu, Guiliang; Xin, Shiying; Geng, Shang; Zheng, Weiwei; Xu, Tianjun; Sun, Yuena
• Is Part Of: Fish & shellfish immunology. Issue 136(2023)
• Publication Date: 2023-05
• Subjects: Fusion gene -- NLRC3-NLRP12 -- NLRs -- Innate immunity -- NF-κB; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108697
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Fusion gene is a new gene formed by the fusion of all or part of the sequences of two genes, it is caused by chromosome translocation, middle deletion or chromosome inversion. Numerous studies in the past have continuously shown that gene fusions are tightly associated with the occurrence and development of various diseases, especially cancer. Many fusion genes have been identified in humans. However, few fusion genes have been identified in fish. In this study, a novel NLRC3-NLRP12 fusion gene was identified in the Miichthys miiuy (miiuy croaker) by quantitative real-time PCR (qRT-PCR), PCR, and Sanger sequencing. This fusion gene is fused by two genes related to NLRs (nucleotide binding domain and oligomerization domain like receptors). We found that the expression of the NLRC3-NLRP12 fusion gene was significantly upregulated after infection with Vibrio anguillarum ( V. anguillarum ) or stimulation with lipopolysaccharide (LPS). In addition, the NLRC3-NLRP12 fusion gene was strongly induced by V. anguillarum infection, peaking within the kidney and liver at 12 h post infection. Further functional experiments showed that overexpression of NLRC3-NLRP12 significantly inhibited nuclear factor kappa-B (NF-κB) activation. This study suggests that the newly discovered NLRC3-NLRP12 fusion genes may play an important role in innate immunity in miiuy croaker. Highlights: The fusion gene NLRC3-NLRP12 was fused by two genes related to the NLRs family. NLRC3-NLRP12 was upregulated after LPS or Vibrio anguillarum treatment in miiuy croaker. NLRC3-NLRP12 can inhibit NF-κB signaling pathway.