Identification of key differentially expressed genes in SARS-CoV-2 using RNA-seq analysis with a systems biology approach. (June 2023)

• Record Type: Journal Article
• Authors: Safarzadeh, Arash; Mahmud Hussen, Bashdar; Taheri, Mohammad; Ghafouri-Fard, Soudeh; Hajiesmaeili, Mohammadreza
• Is Part Of: Cytokine. Volume 166(2023)
• Publication Date: 2023-06
• Subjects: SARS-CoV-2 -- Gene expression -- Immune response; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2023.156187
• Languages: English
• ISSNs: 1043-4666

Abstract

Abstract: COVID-19 is associated with dysregulation of several genes and signaling pathways. Based on the importance of expression profiling in identification of the pathogenesis of COVID-19 and proposing novel therapies for this disorder, we have employed an in silico approach to find differentially expressed genes between COVID-19 patients and healthy controls and their relevance with cellular functions and signaling pathways. We obtained 630 DEmRNAs, including 486 down-regulated DEGs (such as CCL3 and RSAD2) and 144 up-regulated DEGs (such as RHO and IQCA1L), and 15 DElncRNAs, including 9 down-regulated DElncRNAs (such as PELATON and LINC01506) and 6 up-regulated DElncRNAs (such as AJUBA-DT and FALEC). The PPI network of DEGs showed the presence of a number immune-related genes such as those coding for HLA molecules and interferon regulatory factors. Taken together, these results highlight the importance of immune-related genes and pathways in the pathogenesis of COVID-19 and suggest novel targets for treatment of this disorder.