Production of N-acetylglucosamine from carbon dioxide by engineering Cupriavidus necator H16. (July 2023)

• Record Type: Journal Article
• Authors: Wang, Xiaolu; Chang, Fangfang; Wang, Tingting; Luo, Huiying; Su, Xiaoyun; Tu, Tao; Wang, Yuan; Bai, Yingguo; Qin, Xing; Zhang, Honglian; Wang, Yaru; Yao, Bin; Huang, Huoqing; Zhang, Jie
• Is Part Of: Bioresource technology. Volume 379(2023)
• Publication Date: 2023-07
• Subjects: Metabolic engineering -- N-acetylglucosamine -- CO2 -- Cupriavidus necator; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2023.129024
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Conversion of CO2 to GlcNAc was achieved for the first time by engineered C . necator . GlcNAc biosynthetic pathway was constructed by expression of Cegna1 *. PHB synthesis pathway and ED pathway disruption increased the production of GlcNAc. The maximum GlcNAc titer from CO2 reached 75.3 mg/L. Abstract: The conversion of CO2 into valuable bioactive substances using synthetic biological techniques is a potential approach for mitigating the greenhouse effect. Here, the engineering of C . necator H16 to produce N -acetylglucosamine (GlcNAc) from CO2 is reported. First, GlcNAc importation and intracellular metabolic pathways were disrupted by the deletion of nagF, nagE, nagC, nagA and nagB genes. Second, the GlcNAc-6-phosphate N -acetyltransferase gene ( gna1 ) was screened. A GlcNAc-producing strain was constructed by overexpressing a mutant gna1 from Caenorhabditis elegans . A further increase in GlcNAc production was achieved by disrupting poly(3-hydroxybutyrate) biosynthesis and the Entner–Doudoroff pathways. The maximum GlcNAc titers were 199.9 and 566.3 mg/L for fructose and glycerol, respectively. Finally, the best strain achieved a GlcNAc titer of 75.3 mg/L in autotrophic fermentation. This study demonstrated a conversion of CO2 to GlcNAc, thereby providing a feasible approach for the biosynthesis of various bioactive chemicals from CO2 under normal conditions..