Reversible Photocontrol of Microtubule Stability by Spiropyran‐Conjugated Tau‐Derived Peptides. (29th March 2023)

• Record Type: Journal Article
• Authors: Inaba, Hiroshi; Sakaguchi, Minamo; Watari, Soei; Ogawa, Shigesaburo; Kabir, Arif Md. Rashedul; Kakugo, Akira; Sada, Kazuki; Matsuura, Kazunori
• Is Part Of: Chembiochem. Volume 24:Number 8(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-03-29
• Subjects: microtubules -- photoisomerization -- spiropyran -- supramolecular chemistry -- Tau-derived peptide; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202200782
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Spatiotemporal modulation of microtubules by light has become an important aspect of the biological and nanotechnological applications of microtubules. We previously developed a Tau‐derived peptide as a binding unit to the inside of microtubules. Here, we conjugated the Tau‐derived peptide to spiropyran, which is reversibly converted to merocyanine by light, as a reversible photocontrol system to stabilize microtubules. Among the synthesized peptides with spiropyran/merocyanine at different positions, several peptides were bound to the inside of microtubules and stabilized the structures of microtubules. The peptide with spiropyran at the N‐terminus induced polymerization and stabilization of microtubules, whereas the same peptide with the merocyanine form did not exert these effects. Reversible formation of microtubules/tubulin aggregates was achieved using the peptide with spiropyran conjugated at the N‐terminus and irradiation with UV and visible light. Spiropyran‐conjugated Tau‐derived peptides would be useful for spatiotemporal modulation of microtubule stability through reversible photocontrol of binding. Abstract : A system to photocontrol stability of microtubules was developed by conjugating spiropyran to a Tau‐derived peptide, a binding unit to the inside of microtubules. One peptide with spiropyran at the N‐terminus stabilized microtubules, whereas the same peptide with the merocyanine form did not exert the effect. Reversible structural change of microtubules was achieved by photoisomerization of the peptide by irradiation of UV and visible light.