Tahibacter harae sp. nov., isolated from pig farm soil in Guangdong, PR China. Issue 4 (21st April 2023)

• Record Type: Journal Article
• Authors: Chen, Haixin; Zhou, Yang; Yao, Qing; Jiang, Hongxia; Zhu, Honghui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-21
• Subjects: Pig farm soil -- polyphasic taxonomy -- Tahibacter -- strain P2KT; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005852
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : In the process of exploring the microbial diversity of pig farms, a Gram-stain-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated P2K T, was isolated from soil sample collected at a pig farm, Guangdong Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P2K T belonged to the genus Tahibacter, with the highest sequence similarity to Tahibacter aquaticus PYM5-11 T (98.6%) and Tahibacter caeni BUT-6 T (98.3 %). The genome size of strain P2K T was 6.0 Mb with a DNA G+C content of 68.3 mol%. Average nucleotide identity values between strain P2K T and the type strains of the genus Tahibacter were 81.1–81.6 %. The digital DNA–DNA hybridization values between P2K T and these relative species were 24.5–25.6%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, an unknown aminolipids, two unknown lipids and three unknown phospholipids. The major respiratory quinone of strain P2K T was ubiquinone Q-8, and the main fatty acids (>10.0 % of total fatty acids) of strain P2K T were iso-C15:0, iso-C16:0 and summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9 c ). Based on phenotypic and genotypic data, strain P2K T represents a novel species within the genus Tahibacter, for which the name Tahibacter harae sp. nov. is proposed, with the type strain P2K T (=GDMCC 1.3107 T =JCM 35231 T ).