Chryseobacterium oryctis sp. nov., isolated from the gut of the beetle Oryctes rhinoceros, and Chryseobacterium kimseyorum sp. nov., isolated from a stick insect rearing cage. Issue 4 (19th April 2023)

• Record Type: Journal Article
• Authors: Shelomi, Matan; Han, Chiao-Jung; Chen, Wen-Ming; Chen, Hsin-Kuang; Liaw, Shwu-Jen; Mühle, Estelle; Clermont, Dominique
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-19
• Subjects: 16S -- ANI -- Chryseobacterium -- rpoB -- taxonomic description; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005813
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two strains of Chryseobacterium identified from different experiments are proposed to represent new species. Strain WLa1L2M3 T was isolated from the digestive tract of an Oryctes rhinoceros beetle larva. Strain 09-1422 T was isolated from a cage housing the stick insect Eurycantha calcarata . Sequence analysis of the 16S rRNA and rpoB genes found both strains to be similar but not identical to other Chryseobacterium species. Whole-genome sequencing suggested the isolates represent new species, with average nucleotide identity values ranging from 74.6 to 80.5 %. Genome-to-genome distance calculations produced values below 25.3 %, and digital DNA–DNA hybridization values were 13.7–29.9 %, all suggesting they are distinct species. The genomic DNA G+C content of WLa1L2M3 T is approximately 32.53 %, and of 09-1422 T is approximately 35.89 %. The predominant cellular fatty acids of strain WLa1L2M3 T are C15 : 0 iso, summed feature 9 (C16 : 0 10OH or C17 : 1 iso ω6 c ), C17 : 0 iso 3OH, summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6c), C15 : 0 iso 3OH, C15 : 0 anteiso and C13 : 0 iso, and those of strain 09-1422 T are C15 : 0 iso, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6 c ), C17 : 0 iso 3OH, C15 : 0 anteiso, C15 : 0 iso 3OH, C16 : 1 ω7 c, C17 : 0 2OH and C18 : 0 . In addition, physiological and biochemical tests revealed phenotypic differences from related Chryseobacterium type strains. These cumulative data indicate that the two strains represent novel species of the genus Chryseobacterium for which the names Chryseobacterium oryctis sp. nov. and Chryseobacterium kimseyorum sp. nov. are proposed with WLa1L2M3 T (=BCRC 81350 T =JCM 35215 T =CIP 112035 T ) and 09-1422 T (=UCDFST 09-1422 T =BCRC 81359 T =CIP 112165 T ), as type strains, respectively.