Hypoxia regulates tumour characteristic RNA modifications in ovarian cancers. (7th December 2022)

• Record Type: Journal Article
• Authors: Monoe, Yuya; Miyamoto, Shunsuke; Jingushi, Kentaro; Tanimoto, Masaya; Tanaka, Tomohito; Taniguchi, Kohei; Komura, Kazumasa; Ohmichi, Masahide; Tsujikawa, Kazutake
• Is Part Of: FEBS journal. Volume 290:Number 8(2023)
• Page Start: 2085
• Page End: 2096
• Publication Date: 2022-12-07
• Subjects: hypoxia -- ovarian cancer -- RNA modification -- tumour microenvironment; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.16688
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : Analysis of ovarian cancer tissue and normal ovarian tissue revealed 31 RNA modifications with significant differences observed in cancer tissue compared with normal tissue. Moreover, we found Im and chm5U as characteristic RNA modifications in advanced and platinum‐resistant ovarian cancers, respectively. Considering that these differences in RNA modifications may be due to the intra‐tumour microenvironment, we xenografted the ovarian cancer cell line RMG‐1 to create RMG‐1 tumours and compared them with original RMG‐1 cells. As a result, 14 of the 31 RNA modifications showed marked variations during tumorigenesis. Eight RNA modifications (m2, 2G, t6A, m7G, m5U, m1G, i6A, m6t6A and m1A), which were upregulated in ovarian cancer tissues and in RMG‐1‐xenografted tumour, were also upregulated under hypoxic conditions. RNAseq analysis, using the matched RNA samples analysed for RNA modifications, showed that 2137 genes were highly expressed in ovarian cancer tissues compared with those in normal ovarian tissues. Of these, 134 genes, which were enriched in a gene set belonging to the hypoxia signalling pathway, were positively correlated with the above eight RNA modifications. These results suggest that the tumour microenvironment, including hypoxia, is important for cancer characteristic RNA modifications. Abstract : Analysis of ovarian cancer tissue and normal ovarian tissue revealed 31 RNA modifications with significant differences observed in cancer tissue compared with normal tissue. Eight RNA modifications (m2, 2G, t6A, m7G, m5U, m1G, i6A, m6t6A and m1A), which were upregulated in ovarian cancer tissues were also upregulated under hypoxic conditions. These results suggest that the tumour microenvironment, including hypoxia, is important for cancer characteristic RNA modifications.