An In Situ Assembled Trapping Gel Repairs Spinal Cord Injury by Capturing Glutamate and Free Calcium Ions. Issue 16 (22nd January 2023)

• Record Type: Journal Article
• Authors: Huang, Ying; Wang, Jialun; Yue, Chunyan; Wang, Ran; Guo, Qiyuan; Wang, Tingyu; Wang, Daojuan; Dong, Hong; Hu, Yiqiao; Tao, Gaojian; Li, Xihan
• Is Part Of: Small. Volume 19:Issue 16(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-22
• Subjects: calcium ions -- gel -- glutamate -- glutamate decarboxylase -- pluronic F‐127 -- sodium alginate -- spinal cord injuries; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202206229
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Spinal cord injury (SCI) can lead to devastating autonomic dysfunction. One of the most challenging issues for functional repair in SCI is the secondary damage caused by the increased release of glutamate and free Ca 2+ from injured cells. Here, an in situ assembled trapping gel (PF‐SA‐GAD) is developed to sweep glutamate and Ca 2+, promoting SCI repair. The hydrogel solution is a mixture of recombinant glutamate decarboxylase 67 (rGAD67) protein, sodium alginate (SA), and pluronic F‐127 (PF‐127). After intrathecal administration, temperature‐sensitive PF‐127 promoted in situ gelation. Glutamate (Glu) is captured and decarboxylated by rGAD67 into γ‐aminobutyric acid (GABA). SA reacted with the free Ca 2+ to generate gellable calcium alginate. Thereby, this in situ trapping gel retarded secondary neuron injury caused by Glu and free Ca 2+ during SCI. In rat models of SCI, PF‐SA‐GAD reduces the lesion volume and inflammatory response after SCI, restores the motor function of rats with SCI. Together, the in situ assembled trapping gel is a long‐term effective and minimally invasive sweeper for the direct elimination of glutamate and Ca 2+ from injury lesions and can be a novel strategy for SCI repair by preventing secondary injury. Abstract : In situ assembled capture gel can repair spinal cord injury (SCI) by capturing glutamate (Glu) and Ca 2+ . rGAD67 and sodium alginate (SA) are assembled into temperature‐responsive hydrogels. rGAD67 enables Glu to be captured and transformed into GABA. Free Ca 2+ exchanges with Na + in SA to form calcium alginate. The inflammatory reaction is reduced, and SCI repair is accelerated.