Effect of titanium-doped bioactive glass on poly(2-hydroxyethyl methacrylate) hydrogel composites: Bioactivity, intermediate water, cell proliferation, and adhesion force. (1st May 2023)

• Record Type: Journal Article
• Authors: Mabrouk, Mostafa; Beherei, Hanan H.; Shiomoto, Shohei; Tanaka, Yukiko; Osama, Lamyaa; Tanaka, Masaru
• Is Part Of: Ceramics international. Volume 49(2023)Part A
• Page Start: 13469
• Page End: 13481
• Publication Date: 2023-05-01
• Subjects: Intermediate water -- Bioactive glass -- PHEMA hydrogel -- Bone repair; Ceramics -- Periodicals; Céramique industrielle -- Périodiques; Ceramics; Periodicals; Electronic journals; 666
• DOI: 10.1016/j.ceramint.2022.12.221
• Languages: English
• ISSNs: 0272-8842

Abstract

Abstract: The intermediate water (IW) concept has been hypothesized to predict the biocompatibility of polymers and inorganic materials. This study, for the first time, prepared the composite biomaterials of poly(2-hydroxyethyl methacrylate) (PHEMA)/titanium-doped bioactive glasses (BGT) for bone regeneration by applying the IW concept. The homogeneous distribution of BGT and the mechanical stability of the composites were confirmed using X-ray diffraction, infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectrometry, and rheological characterization. In vitro tests with immersion in simulated body fluid showed that titanium doping improved the biomineralization properties. The IW level ranged from 10.4 wt% with 0% titanium doped to 25.6 wt% with 7.5% titanium doped. Cell viability tests on osteosarcoma cells revealed that the growth rate increased with increasing titanium concentration, reaching up to 150%. Based on single-cell force spectroscopy, an appropriate titanium concentration for cell adhesion was estimated to be 5%. The hydrated PHEMA gels with 5% BGT exhibited a high IW level of 19.3% and less hydrophilic wettability than the gels without titanium or with 10% BGT. The IW concept proved to be useful for predicting the bone regeneration potential on the interfaces of the PHEMA/BGT composite hydrogels.