Accumulation and cross-linkage of β-1, 3/1, 6-glucan lead to loss of basal stipe cell wall extensibility in mushroom Coprinopsis cinerea. (1st May 2021)

• Record Type: Journal Article
• Authors: Li, Maomao; Bi, Jingjing; Bai, Yang; Kang, Liqin; Duan, Baiyun; Liu, Zhonghua; Yuan, Sheng
• Is Part Of: Carbohydrate polymers. Volume 259(2021)
• Publication Date: 2021-05-01
• Subjects: β-1, 6-branched β-1, 3-glucan -- Chitin -- Wall extension -- Wall maturation -- Stipe elongation; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.117743
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Endo-β-1, 3-glucanase (ENG) could restore mature basal stipe wall extensibility. ENG-pretreated basal stipe walls could be induced to extend by chitinase. ENG released more glucose from the basal stipe walls than the apical stipe walls. Products released from basal stipe by ENG were consistent with that of β-1, 3/1, 6-glucan. β-1, 3/1, 6-Glucan more accumulated and cross-linked in basal stipe than apical stipe Abstract: The mature basal stipe of mushroom Coprinopsis cinerea loses wall extensibility. We found that an endo-β-1, 3-glucanase ENG from C. cinerea could restore mature basal stipe wall extensibility via pretreatment such that the ENG-pretreated basal stipe walls could be induced to extend by chitinase ChiIII. ENG pretreatment released glucose, laminaribiose, and 3-O-D-gentiobiose-D-glucose from the basal stipe walls, consistent with ENG-digested products of β-1, 6-branched β-1, 3-glucan. Different effects of endo-β-1, 3-glucanase ENG and exo-β-1, 3-glucanase EXG pretreatment on the structure, amount and ratio (β-1, 3-glucoside bonds to β-1, 6-glucoside bonds) of products from the basal stipe and the apical stipe cell walls, respectively, and on the cell wall extensibility and the cell wall ultra-architecture of the basal stipes were analyzed. All results demonstrate that the more accumulation and cross-linkage of β-1, 6-branched β-1, 3-glucan with wall maturation lead to loss of wall extensibility of the basal stipe regions compared to the apical stipe cell walls.