Dysregulation of HNF1B/Clusterin axis enhances disease progression in a highly aggressive subset of pancreatic cancer patients. (25th November 2022)

• Record Type: Journal Article
• Authors: Yang, Shouhui; Tang, Wei; Azizian, Azadeh; Gaedcke, Jochen; Ströbel, Philipp; Wang, Limin; Cawley, Helen; Ohara, Yuuki; Valenzuela, Paloma; Zhang, Lin; Lal, Trisha; Sinha, Sanju; Rupin, Eythan; Hanna, Nader; Ghadimi, B Michael; Hussain, S Perwez
• Is Part Of: Carcinogenesis. Volume 43:Number 12(2022)
• Page Start: 1198
• Page End: 1210
• Publication Date: 2022-11-25
• Subjects: Carcinogenesis -- Periodicals; Cancer -- Genetic aspects -- Periodicals; Cancer -- Prevention -- Periodicals; Cancer -- Periodicals; 616.994071
• DOI: 10.1093/carcin/bgac092
• Languages: English
• ISSNs: 0143-3334

Abstract

Abstract: Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy and is largely refractory to available treatments. Identifying key pathways associated with disease aggressiveness and therapeutic resistance may characterize candidate targets to improve patient outcomes. We used a strategy of examining the tumors from a subset of PDAC patient cohorts with the worst survival to understand the underlying mechanisms of aggressive disease progression and to identify candidate molecular targets with potential therapeutic significance. Non-negative matrix factorization (NMF) clustering, using gene expression profile, revealed three patient subsets. A 142-gene signature specific to the subset with the worst patient survival, predicted prognosis and stratified patients with significantly different survival in the test and validation cohorts. Gene-network and pathway analysis of the 142-gene signature revealed dysregulation of Clusterin (CLU) in the most aggressive patient subset in our patient cohort. Hepatocyte nuclear factor 1 b (HNF1B) positively regulated CLU, and a lower expression of HNF1B and CLU was associated with poor patient survival. Mechanistic and functional analyses revealed that CLU inhibits proliferation, 3D spheroid growth, invasiveness and epithelial-to-mesenchymal transition (EMT) in pancreatic cancer cell lines. Mechanistically, CLU enhanced proteasomal degradation of EMT-regulator, ZEB1. In addition, orthotopic transplant of CLU-expressing pancreatic cancer cells reduced tumor growth in mice. Furthermore, CLU enhanced sensitivity of pancreatic cancer cells representing aggressive patient subset, to the chemotherapeutic drug gemcitabine. Taken together, HNF1B/CLU axis negatively regulates pancreatic cancer progression and may potentially be useful in designing novel strategies to attenuate disease progression in PDAC patients. Abstract : This study illustrates a novel HNF1B/Clusterin axis negatively regulating disease aggressiveness by enhancing proteasomal degradation of ZEB1 and regulating EMT in a highly aggressive subset of PDAC. Therapies targeting HNF1B/Clusterin may provide a novel approach to potentially improve disease outcome in PDAC patients. Graphical abstract: