Cytochrome P450 2D6 biosensor for perphenazine based on multi-walled carbon nanotube/ionic liquid and tetrathiafulvalene-tetracyanoquinodimethane salt/ionic liquid gels. (20th January 2023)

• Record Type: Journal Article
• Authors: Shu, Ting; Wang, Junyi; Li, Xiatong; Wang, Xiaobo; Wang, Shi
• Is Part Of: Electrochimica acta. Volume 439(2023)
• Publication Date: 2023-01-20
• Subjects: Cytochrome P450 2D6 -- Tetrathiafulvalene-tetracyanoquinodimethane -- Ionic liquid -- Multi-walled carbon nanotube -- Perphenazine -- biosensor; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2022.141656
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: A new approach to prepare TTF–TCNQ/IL gel. MWNT/IL gel is used as the matrix of TTF–TCNQ/IL gel. The synergetic effect of MWNT/IL and TTF–TCNQ/IL promote the catalysis of CYP2D6. Low detection limit for perphenazine. Abstract: A cytochrome P450 2D6 (CYP2D6) biosensor was developed with the incorporation of multi-walled carbon nanotube/ionic liquid and tetrathiafulvalene-tetracyanoquinodimethane salt/ionic liquid (MWNT/IL-[TTF–TCNQ]/IL) gels. The MWNT/IL-[TTF–TCNQ]/IL gels were characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, and scanning electron microscopy. Utilizing the excellent stability of the MWNT/IL gel and the unique structure of the [TTF–TCNQ]/IL gel, CYP2D6 was immobilized on the screen-printed electrode with high bioactivity. Moreover, the CYP2D6 catalytic effect was highly promoted upon application of MWNT/IL-[TTF–TCNQ]/IL gels as the mediating system. The proposed MWNT/IL-[TTF–TCNQ]/IL gel-based CYP2D6 biosensor was used to detect perphenazine via voltammetry and amperometry. The calibration of perphenazine ranged from 6 × 10 −8 to 1.6 × 10 −5 M with a detection limit of 2.05 × 10 −8 M. It is expected that the MWNT/IL-[TTF–TCNQ]/IL gel-based CYP2D6 biosensor opens new avenues for promising advances in the analysis of drugs. Graphical abstract: Image, graphical abstract