Inhibition of platelet aggregation by extracts and compounds from the leaves of Chilean bean landraces (Phaseolus vulgaris L.). (January 2023)

• Record Type: Journal Article
• Authors: Fuentes, Eduardo; Rodríguez, Lyanne; Méndez, Diego; Alarcón-Espósito, Jazmín; Nina, Nélida; Burgos-Edwards, Alberto; Carrasco, Basilio; Palomo, Iván; Schmeda-Hirschmann, Guillermo
• Is Part Of: Journal of functional foods. Volume 100(2023)
• Publication Date: 2023-01
• Subjects: Phaseolus vulgaris -- Chilean bean landraces -- Anti-platelet aggregation -- Rutin -- Kaempferol 3-glucoside -- Caffeoyl malic acid; ADP Adenosine 5′-diphosphate -- BLE Bean Leaf Extract -- CMA Caffeoyl malic acid -- CVD Cardiovascular disease -- DMSO Dimethyl sulfoxide -- HDL High-density lipoprotein -- HPLC high performance liquid chromatography -- K-3-G Kaempferol 3-O-glucoside -- LDL Low-density lipoprotein -- LOQ limit of quantification -- MeOH methanol -- PRP Platelet Rich Plasma -- PPP Platelet Poor Plasma -- TRAP-6 Thrombin 6 receptor activating peptide; Caffeoyl malic acid (PubChem CID: 6124299) -- Rutin (PubChem CID: 5280805) -- Kaempferol 3-O-β-d-glucoside (PubChem CID: 25203515); Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105388
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Leaf extracts from Chilean bean landraces were assessed for antiplatelet activity. The HPLC-DAD study allowed the identification of caffeoyl malic acid, derivatives of quercetin, and kaempferol. The most active extracts with antiplatelet effect were Frutilla and Sapito landraces. Antiplatelet activity was associated with the content of rutin, caffeoyl malic acid, and kaempferol 3-O-glucoside. Abstract: Leaf extracts from Chilean bean landraces were assessed for antiplatelet activity using different assays in vitro . Lyophilized MeOH:H2 O 7:3 (v/v) and EtOH:H2 O 7:3 (v/v) leaf extracts from the Chilean landraces Coscorron, Frutilla, Magnum, Sapito and Tortola were compared for platelet aggregation effect by turbidimetry and flow cytometry. The HPLC-DAD study allowed the identification of caffeoyl malic acid, derivatives of quercetin and kaempferol. The results showed that the EtOH:H2 O 7:3 extracts have higher antiplatelet capacity, with better effects for the Frutilla and Sapito landraces (IC50 0.38 ± 0.05 and 0.16 ± 0.08 mg/mL, respectively). The Sapito extract inhibited P-selectin expression, CD63 secretion, and concentration-dependent GPIIb/IIIa activation. The activity was associated with the main phenolics content, including rutin, caffeoyl malic acid and kaempferol 3-O-glucoside. The findings add evidence for the potential of bean leaves as greens, affording naturally occurring compounds with health promoting properties, including inhibition of platelet aggregation in vitro .