Membranihabitans maritimus sp. nov., a marine bacterium isolated from deep seawater. Issue 3 (30th March 2023)

• Record Type: Journal Article
• Authors: Guo, Li-Li; Yi, Li-Dong; Wang, Ming-Lei; Qian, Yu-Rong; Xu, Xue-Wei; Fu, Ge-Yi
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 3(2023)
• Publication Date: 2023-03-30
• Subjects: Membranihabitans -- Bacteroidota -- seawater -- whole-genome sequencing -- phylogenetic analysis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005774
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated C281 T, was isolated from seawater sampled at the Marshallese seamount chain. Results of 16S rRNA gene analysis revealed that strain C281 T was most closely related to Membranihabitans marinus CZ-AZ5 T with 92.7 % sequence similarity. Phylogenetic analysis indicated that the new isolate represented a novel species by forming a distinctive lineage within the family Saprospiraceae . The DNA G+C content of strain C281 T was 38.4 mol%. The genome sizes of strain C281 T and the reference strain M. marinus CZ-AZ5 T were 5 962 917 and 5 395 999 bp, respectively. The average nucleotide identity and in silico DNA–DNA hybridization values between strains C281 T and M. marinus CZ-AZ5 T were found to be low (69.3 and 17.6 %, respectively). Different functional genes were found in the genome of strain C281 T, such as CZC CBA, polysaccharide utilization loci and linear azol(in)e-containing peptide cluster coding genes. The NaCl range for growth was 0.5–15.0 %. Positive results were obtained for hydrolysis of Tween 60 and urease. MK-7 was the sole respiratory quinone. The major fatty acids were C16 : 1 ω 6 c and/or C16 : 1 ω 7 c, iso-C15 : 0 and iso-C15 : 1 F. The major polar lipids of strain C281 T were phosphatidylethanolamine, phosphatidylglycerol, two unidentified lipids and five unidentified glycolipids. On the basis of its taxonomic characteristics, the isolate represents a novel species of the genus Membranihabitans, for which the name Membranihabitans maritimus sp. nov. (type strain C281 T =KCTC 92171 T =MCCC M27001 T ) is proposed.