Polyethylenimine‐Grafted Silica Nanoparticles Facilitate Enzymatic Carbon Dioxide Conversion. Issue 5 (2nd February 2023)

• Record Type: Journal Article
• Authors: Liu, Yu; Li, Yu-Tong; Liu, Ling; Bai, Shu; Yu, Lin-Ling; Sun, Yan
• Is Part Of: Chemical engineering & technology. Volume 46:Issue 5(2023)
• Page Start: 858
• Page End: 864
• Publication Date: 2023-02-02
• Subjects: Carbon dioxide conversion -- Enzyme immobilization -- Enzyme mimetics -- Phosphoenolpyruvate carboxylase -- Polyethylenimine; Chemical engineering -- Periodicals; Chemical processes -- Periodicals; 660.05
• DOI: 10.1002/ceat.202200271
• Languages: English
• ISSNs: 0930-7516

Abstract

Abstract: Polyethylenimine (PEI)‐grafted silica nanoparticles were fabricated and characterized for the electrostatic immobilization of a CO2 conversion enzyme. PEI was supposed to serve as a carbonic anhydrase mimic for CO2 capture and conversion to HCO3 − (the optimal substrate of many CO2 conversion enzymes) and was proved to assist phosphoenolpyruvate carboxylase to form a dual‐enzyme cascade system for CO2 conversion. The immobilized enzyme not only presented better thermostability, pH tolerance, and storage stability, but also enhanced the specific activity (three‐ and fourfold for HCO3 − and CO2 as the substrates, respectively), with good reusability and low cost. It was proven that PEI‐grafted nanoparticles are highly efficient nanocarriers for immobilizing CO2 ‐converting enzymes in industrial applications. Abstract : Polyethyleneimine was grafted onto silica nanoparticles (SNP‐PEI) for the electrostatic immobilization of phosphoenolpyruvate carboxylase (PEPCase), a CO2 conversion enzyme. SNP‐PEI facilitated enzymatic CO2 conversion by both increasing the enzyme stability and mimicking carbonic anhydrase to catalyze CO2 turnover to HCO3 −, which is the better substrate of PEPCase.