Αβ T cells replacing dermal and epidermal γδ T cells in Tcrd−/− mice express an MHC‐independent TCR repertoire. Issue 11 (4th September 2021)

• Record Type: Journal Article
• Authors: Binz, Christoph; Bubke, Anja; Sandrock, Inga; Prinz, Immo
• Is Part Of: European journal of immunology. Volume 51:Issue 11(2021)
• Page Start: 2618
• Page End: 2632
• Publication Date: 2021-09-04
• Subjects: DETC -- skin T cells -- Tcrd−/− mouse -- TCR repertoire -- MHC‐independent αβ TCR; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.202149243
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract: The epidermis of mouse skin is usually populated by dendritic epidermal T cells (γδDETC) expressing an invariant Vγ5Vδ1 + TCR. In Tcrd −/− mice, skin‐resident γδDETC are replaced by αβDETC carrying polyclonal αβ TCRs. Although they exhibit a dendritic morphology, αβDETC were reported to be less functional than genuine γδDETC, likely because their TCR is unable to interact with the original TCR ligands of γδDETC. However, the TCR repertoire of those replacement DETC in Tcrd −/− mice might provide clues for understanding the development and selection of canonical γδDETC. Here, we compare the phenotype and TCR repertoires of wild‐type and Tcrd −/− mouse skin T cells. Our data reveal that αβDETC are CD4/CD8 double negative and express an MHC‐independent TCR repertoire. Furthermore, we identify a second MHC‐independent population of CD103 hi CD4/ CD8 double‐negative αβ T cells in the dermis of Tcrd −/− mice. Abstract : By profiling skin T cells of Tcrd −/− mice, we characterize the MHC‐independent TCR‐repertoire of αβ T cells replacing missing γδ T cell in the dermis and epidermis. The results add to the discussion about the ontogeny, selection, and TCR‐specificity of mouse dendritic epidermal T cells (DETCs).