Titelbild: Reversible Markierung in lebenden Zellen mit retro‐konstruierten HaloTags ermöglicht hoch‐ und superauflösende Langzeitbildgebung (Angew. Chem. 18/2023). (13th April 2023)

• Record Type: Journal Article
• Authors: Holtmannspötter, Michael; Wienbeuker, Eike; Dellmann, Timo; Watrinet, Isabelle; Garcia‐Sáez, Ana J.; Johnsson, Kai; Kurre, Rainer; Piehler, Jacob
• Is Part Of: Angewandte Chemie. Volume 135:Number 18(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-04-13
• Subjects: Einzelmolekülverfolgung -- Fluoreszierende Sonden -- Protein-Engineering -- STED-Mikroskopie -- Selbstmarkierende Enzyme; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202303994
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract : Reversible posttranslational fluorescence labeling opens exciting possibilities for advanced and superresolution microscopy techniques. In their Research Article (e202219050 ) Michael Holtmannspötter, Jacob Piehler et al. have engineered HaloTag variants with restored dehalogenase activity, which enzymatically exchange fluorogenic substrates at the second‐to‐minutes time scale. Thus, long‐term time‐lapse imaging with utmost spatial and temporal resolution is achieved with particularly striking performance in single molecule tracking and localization microscopy.