High salt diet‐induced proximal tubular phenotypic changes and sodium‐glucose cotransporter‐2 expression are coordinated by cold shock Y‐box binding protein‐1. Issue 10 (17th September 2021)

• Record Type: Journal Article
• Authors: Bernhardt, Anja; Häberer, Saskia; Xu, JingJing; Damerau, Hannah; Steffen, Johannes; Reichardt, Charlotte; Wolters, Katharina; Steffen, Hannes; Isermann, Berend; Borucki, Katrin; Artelt, Nadine; Endlich, Nicole; Kozyraki, Renata; Brandt, Sabine; Lindquist, Jonathan A.; Mertens, Peter R.
• Is Part Of: FASEB journal. Volume 35:Issue 10(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-17
• Subjects: cold shock protein -- high salt diet -- sodium glucose transporter -- tubular damage; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.202100667RR
• Languages: English
• ISSNs: 0892-6638

Abstract

Abstract: High salt diet (HSD) is a hallmark of blood pressure elevations, weight gain and diabetes onset in the metabolic syndrome. In kidney, compensatory mechanisms are activated to balance salt turnover and maintain homeostasis. Data on the long‐term effects of HSD with respect to tubular cell functions and kidney architecture that exclude confounding indirect blood pressure effects are scarce. Additionally we focus on cold shock Y‐box binding protein‐1 as a tubular cell protective factor. A HSD model (4% NaCl in chow; 1% NaCl in water) was compared to normal salt diet (NSD, standard chow) over 16 months using wild type mice and an inducible conditional whole body knockout for cold shock Y‐box binding protein‐1 (BL6J/N, Ybx1 ). HSD induced no difference in blood pressure over 16 months, comparing NSD/HSD and Ybx1 wild type/knockout. Nevertheless, marked phenotypic changes were detected. Glucosuria and subnephrotic albuminuria ensued in wild type animals under HSD, which subsided in Ybx1 ‐deficient animals. At the same time megalin receptors were upregulated. The sodium‐glucose cotransporter‐2 (SGLT2) was completely downregulated in wild type HSD animals that developed glucosuria. In Ybx1 knockouts, expression of AQP1 and SGLT2 was maintained under HSD; proximal tubular widening and glomerular tubularization developed. Concurrently, amino aciduria of neutral and hydrophobic amino acids was seen. In vitro translation confirmed that YB‐1 translationally represses Sglt2 transcripts. Our data reveal profound effects of HSD primarily within glomeruli and proximal tubular segments. YB‐1 is regulated by HSD and orchestrates HSD‐dependent changes; notably, sets reabsorption thresholds for amino acids, proteins and glucose.