Pumpless deterministic lateral displacement separation using a paper capillary wick. Issue 8 (21st March 2023)

• Record Type: Journal Article
• Authors: Aghajanloo, Behrouz; Ejeian, Fatemeh; Frascella, Francesca; Marasso, Simone L.; Cocuzza, Matteo; Tehrani, Alireza Fadaei; Nasr Esfahani, Mohammad Hossein; Inglis, David W.
• Is Part Of: Lab on a chip. Volume 23:Issue 8(2023)
• Page Start: 2106
• Page End: 2112
• Publication Date: 2023-03-21
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d3lc00039g
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : We demonstrate a highly efficient DLD separation device and process that is driven by a paper wick yet allows direct collection of products from reservoirs. Abstract : Deterministic lateral displacement (DLD) is a passive separation method that separates particles by hydrodynamic size. This label-free method is a promising technique for cell separation because of its high size resolution and insensitivity to flow rate. Development of capillary-driven microfluidic technologies allows microfluidic devices to be operated without any external power for fluid pumping, lowering their total cost and complexity. Herein, we develop and test a DLD-based particle and cell sorting method that is driven entirely by capillary pressure. We show microchip self-filling, flow focusing, flow stability, and capture of separated particles. We achieve separation efficiency of 92% for particle–particle separation and more than 99% efficiency for cell–particle separation. The high performance of driven flow and separation along with simplicity of the operation and setup make it a valuable candidate for point-of-care devices.