Preferential disruption of E. coli biofilm via ratiometric detection and targeting of extracellular matrix using graphene-oxide-conjugated red-emitting fluorescent copper nanoclusters. Issue 4 (3rd March 2023)

• Record Type: Journal Article
• Authors: Sharma, Chandni; Shukla, Ashish K.; Verma, Mohini; Bathla, Manik; Acharya, Amitabha
• Is Part Of: Environmental science. Volume 10:Issue 4(2023)
• Page Start: 1077
• Page End: 1095
• Publication Date: 2023-03-03
• Subjects: Environmental sciences -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d2en00899h
• Languages: English
• ISSNs: 2051-8153

Abstract

Abstract : Graphene oxide conjugated red emitting fluorescent copper nanoassembly for extracellular matrix fibril detection and disruption of bacterial biofilm. Abstract : The emerging demand for antibiofilm coating nanostructures has led to the design of graphene oxide (GO) hosted copper nanoclusters (CuNCs). In this study, we have developed red fluorescence emitting Cu@GO@CTAB nanostructures. The size of the CuNCs in Cu@GO@CTAB was found to be ∼16.7 ± 2.1 nm. The developed nanoassembly showed dual fluorescence emission maxima at ∼474 and ∼645 nm and revealed more promising antibiofilm potential for E. coli (MBIC50 ∼74.1 ± 5.1 μg mL −1 ) as compared to S. aureus . Interestingly, nanomaterials (NMs) have shown ratiometric interaction towards E. coli isolated extracellular matrix fibrils wherein an isoemissive point was obtained at ∼569 nm. The limit of detection for extracellular matrix fibrils from E. coli and lipopolysaccharide (LPS) was found to be ∼331.1 ± 12.3 and 9.47 ± 1.12 μg mL −1, respectively. Confocal studies suggested that Cu@GO@CTAB NMs can preferentially penetrate and reduce the overall thickness of E. coli biofilm. Motility assays suggested that the developed nanostructures restricted the motility of E. coli and S. aureus . Mechanistic assays for outer and inner membranes suggested that bacterial membrane disruption induced cell death. A displacement assay using BODIPY TR cadaverine (BC) revealed that the prepared NM showed strong binding to LPS of E. coli O26:B6. These studies may pave the path for developing antibacterial and antibiofilm coating agents.