The correlation between denaturation of glyceraldehyde-3-phosphate dehydrogenase and inactivation of Saccharomyces pastorianus cells by pressurized carbon dioxide microbubbles. (10th April 2023)

• Record Type: Journal Article
• Authors: Takahashi, Konomi; Kobayashi, Fumiyuki; Narai-Kanayama, Asako; Odake, Sachiko
• Is Part Of: Letters in applied microbiology. Volume 76:Number 4(2023)
• Publication Date: 2023-04-10
• Subjects: denaturation -- glyceraldehyde-3-phosphate dehydrogenase -- inactivation -- pressurized carbon dioxide microbubbles -- Saccharomyces pastorianus; Microbiology -- Periodicals; 660.62
• DOI: 10.1093/lambio/ovad043
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: For the purpose of clarifying the relationship between pasteurization and inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Saccharomyces pastorianus cells induced by pressurized carbon dioxide microbubbles (CO2 MB) treatment, a storage test of S. pastorianus cells after CO2 MB treatment was conducted to ascertain their recovery, and the treatment condition in the inactivation of GAPDH in S. pastorianus cells by CO2 MB was investigated. Each population of S. pastorianus for 48, 96, and 144 h at 25°C was decreased significantly by CO2 MB treatment at 35°C for 3 min (MB35-3 and MB35-5) or at 40°C and 45°C for 1 and 3 min (MB40-1, MB40-3, and MB45-1). In the storage test, recovery of treated cells was not observed after storage for 144 h at 25°C. The denaturation of GAPDH in the S. pastorianus cells caused by the same treatment as the storage test was detected by using sodium dodecyl sulphate polyacrylamide gel electrophoresis. While the activities at MB35-1, MB35-3, and MB40-1 were significantly higher than those at non-treatment, and those at MB35-5, MB40-3, and MB45-1 were lower. Therefore, GAPDH denaturation, but not the activity, was associated with the inactivation of S. pastorianus cells.